Coupling S-adenosylmethionine–dependent methylation to growth: Design and uses.
In: PLoS Biology, Jg. 17 (2019-03-11), Heft 3, S. 1-13
Online
academicJournal
Zugriff:
We present a selection design that couples S-adenosylmethionine–dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated. [ABSTRACT FROM AUTHOR]
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Coupling S-adenosylmethionine–dependent methylation to growth: Design and uses.
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Autor/in / Beteiligte Person: | Luo, Hao ; Hansen, Anne Sofie L. ; Yang, Lei ; Schneider, Konstantin ; Kristensen, Mette ; Christensen, Ulla ; Christensen, Hanne B. ; Du, Bin ; Özdemir, Emre ; Feist, Adam M. ; Keasling, Jay D. ; Jensen, Michael K. ; Herrgård, Markus J. ; Palsson, Bernhard O. |
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Zeitschrift: | PLoS Biology, Jg. 17 (2019-03-11), Heft 3, S. 1-13 |
Veröffentlichung: | 2019 |
Medientyp: | academicJournal |
ISSN: | 1544-9173 (print) |
DOI: | 10.1371/journal.pbio.2007050 |
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