A preliminary study on the application of PspA as a carrier for group A meningococcal polysaccharide.
In: PLoS ONE, Jg. 14 (2019-07-10), Heft 7, S. 1-12
Online
academicJournal
Zugriff:
This study aimed to explore the feasibility of pneumococcal surface protein A (PspA) as a carrier protein. Three recombinant pneumococcal surface proteins from three different clades were expressed by the prokaryotic expression system and conjugated to group A meningococcal polysaccharide (GAMP) to generate three polysaccharide-protein conjugates. The conjugates, unconjugated proteins, GAMP, and GAMP-TT vaccine bulk (used as positive control) were immunized into mice, and their immune effects were assessed by the methods of enzyme-linked immunosorbent assay (ELISA), flow cytometry (FCM), and serum bactericidal assay (SBA). The results showed that the polysaccharide-protein conjugates could produce higher levels of anti-GAMP IgG titers (P < 0.05), higher ratios of Th1/Th2 (P < 0.05), and higher levels of serum bactericidal activity (P < 0.05), compared with the unconjugated GAMP. The conjugation of PspAs to GAMP also enhanced the anti-PspA responses compared with unconjugated PspAs except for PspA3. In conclusion, the results indicated that the three PspAs were appropriate carrier proteins, as demonstrated by the characteristics of T-cell dependent responses to the GAMP, and might protect against group A of epidemic cerebrospinal meningitis. [ABSTRACT FROM AUTHOR]
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A preliminary study on the application of PspA as a carrier for group A meningococcal polysaccharide.
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Autor/in / Beteiligte Person: | Wang, Lichan ; Tan, Yajun ; Wei, Chen ; Zhang, Huajie ; Luo, Peng ; Zhang, Shumin ; Ma, Xiao |
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Zeitschrift: | PLoS ONE, Jg. 14 (2019-07-10), Heft 7, S. 1-12 |
Veröffentlichung: | 2019 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 (print) |
DOI: | 10.1371/journal.pone.0218427 |
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