High specificity and sensitivity of Zika EDIII-based ELISA diagnosis highlighted by a large human reference panel.
In: PLoS Neglected Tropical Diseases, Jg. 13 (2019-09-20), Heft 9, S. 1-19
Online
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Zugriff:
Background: Zika virus (ZIKV) and Dengue virus (DENV) are often co-endemic. The high protein-sequence homology of flaviviruses renders IgG induced by and directed against them highly cross-reactive against their antigen(s), as observed on a large set of sera, leading to poorly reliable sero-diagnosis. Methods: We selected Domain III of the ZIKV Envelope (ZEDIII) sequence, which is virus specific. This recombinant domain was expressed and purified for the specific detection of ZEDIII-induced IgG by ELISA from ZIKV-RT-PCR-positive, ZIKV-IgM-positive, flavivirus-positive but ZIKV-negative, or flavivirus-negative sera. We also assessed the reactivity of ZEDIII-specific human antibodies against EDIII of DENV serotype 4 (D4EDIII) as a specific control. Sera from ZEDIII-immunized mice were also tested. Results: Cross-reactivity of IgG from 5,600 sera against total inactivated DENV or ZIKV was high (71.0% [69.1; 72.2]), whereas the specificity and sensitivity calculated using a representative cohort (242 sera) reached 90% [84.0; 95.8] and 92% [84.5; 99.5], respectively, using a ZEDIII-based ELISA. Moreover, purified human IgG against D2EDIII or D4EDIII did not bind to ZEDIII and we observed no D4EDIII reactivity with ZIKV-induced mouse polyclonal IgGs. Conclusions: We developed a ZEDIII-based ELISA that can discriminate between past or current DENV and ZIKV infections, allowing the detection of a serological scar from other flaviviruses. This could be used to confirm exposure of pregnant women or to follow the spread of an endemic disease. [ABSTRACT FROM AUTHOR]
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High specificity and sensitivity of Zika EDIII-based ELISA diagnosis highlighted by a large human reference panel.
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Autor/in / Beteiligte Person: | Denis, Jessica ; Attoumani, Sarah ; Gravier, Patrick ; Tenebray, Bernard ; Garnier, Annabelle ; Briolant, Sébastien ; de Laval, Franck ; Chastres, Véronique ; Grard, Gilda ; Leparc-Goffart, Isabelle ; Coutard, Bruno ; Badaut, Cyril |
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Zeitschrift: | PLoS Neglected Tropical Diseases, Jg. 13 (2019-09-20), Heft 9, S. 1-19 |
Veröffentlichung: | 2019 |
Medientyp: | academicJournal |
ISSN: | 1935-2727 (print) |
DOI: | 10.1371/journal.pntd.0007747 |
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