A novel transcription factor UvCGBP1 regulates development and virulence of rice false smut fungus Ustilaginoidea virens.
In: Virulence, Jg. 12 (2021-12-01), Heft 1, S. 1563-1579
Online
academicJournal
Zugriff:
Ustilaginoidea virens, causing rice false smut (RFS) is an economically important ascomycetous fungal pathogen distributed in rice-growing regions worldwide. Here, we identified a novel transcription factor UvCGBP1 (Cutinase G-box binding protein) from this fungus, which is unique to ascomycetes. Deletion of UvCGBP1 affected development and virulence of U. virens. A total of 865 downstream target genes of UvCGBP1 was identified using ChIP-seq and the most significant KEGG enriched functional pathway was the MAPK signaling pathway. Approximately 36% of target genes contain the AGGGG (G-box) motif in their promoter. Among the targets, deletion of UvCGBP1 affected transcriptional and translational levels of UvPmk1 and UvSlt2, both of which were important in virulence. ChIP-qPCR, yeast one-hybrid and EMSA confirmed that UvCGBP1 can bind the promoter of UvPmk1 or UvSlt2. Overexpression of UvPmk1 in the ∆UvCGBP1-33 mutant restored partially its virulence and hyphae growth, indicating that UvCGBP1 could function via the MAPK pathway to regulate fungal virulence. Taken together, this study uncovered a novel regulatory mechanism of fungal virulence linking the MAPK pathway mediated by a G-box binding transcription factor, UvCGBP1. [ABSTRACT FROM AUTHOR]
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A novel transcription factor UvCGBP1 regulates development and virulence of rice false smut fungus Ustilaginoidea virens.
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Autor/in / Beteiligte Person: | Chen, Xiaoyang ; Li, Pingping ; Liu, Hao ; Chen, Xiaolin ; Huang, Junbin ; Luo, Chaoxi ; Li, Guotian ; Hsiang, Tom ; Collinge, David B. ; Zheng, Lu |
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Zeitschrift: | Virulence, Jg. 12 (2021-12-01), Heft 1, S. 1563-1579 |
Veröffentlichung: | 2021 |
Medientyp: | academicJournal |
ISSN: | 2150-5594 (print) |
DOI: | 10.1080/21505594.2021.1936768 |
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