Influence of encapsulated sodium alginates and pectin on selenium nanoparticles and efficient cardioprotective effect in C2C12 cell line.
In: Journal of Nanoparticle Research, Jg. 26 (2024-03-01), Heft 3, S. 1-16
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Zugriff:
Selenium nanoparticles (SeNPs) have recently garnered substantial attention in drug delivery due to their unique properties. The present study focused on synthesizing SeNPs, utilizing sodium selenite as a template and ascorbic acid as the reducing agent. This was followed by sodium alginate-decorated selenium nanoparticles (SA-SeNPs) and pectin-decorated selenium nanoparticles (PET-SeNPs). These nanoparticles were subjected to UV–vis spectrophotometry, Fourier infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman spectroscopy (RS), and dynamic light scattering (DLS) for characterization. The UV–vis spectroscopy results indicate a peak absorption spectrum at 266 nm for SeNPs, SA-SeNPs, and PET-SeNPs. The FTIR spectrum of SeNPs, SA-SeNPs, and PET-SeNPs was recorded within the wavenumber range of 400–4000 cm−1. XRD analysis confirmed the crystalline nature of SeNPs and SA-SeNPs, while PET-SeNPs were amorphous. SEM analysis revealed spherical morphology for SeNPs, spherical fibrous cross-link for SA-SeNPs, and cubic rod-shaped morphology for PET-SeNPs. Raman's spectroscopy identified characteristic vibrations of monoclinic selenium in SeNPs, SA-SeNPs, and PET-SeNPs. DLS analysis indicated an average particle size distribution ranging from 140 to 192 nm, with a zeta potential value signifying the nanoparticles' excellent stability. The antioxidant properties of SeNPs, SA-SeNPs, and PET-SeNPs via the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay indicated significantly higher scavenging activity for SA-SeNPs (72%) and PET-SeNPs (64%) compared to pure SeNPs (61%). Furthermore, cytotoxicity experiments on the C2C12 cell line showed reductions in cell death at 10 µg/mL concentrations of 16.3%, 11.8%, and 6.7% for SeNPs, SA-SeNPs, and PET-SeNPs, respectively. These results underscore the potential applications of PET-SeNPs and SA-SeNPs in diverse biomedical domains, including their use as antioxidant agents, drug delivery systems, and therapeutic agents for cardiovascular issues. [ABSTRACT FROM AUTHOR]
Titel: |
Influence of encapsulated sodium alginates and pectin on selenium nanoparticles and efficient cardioprotective effect in C2C12 cell line.
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Autor/in / Beteiligte Person: | Naveenkumar, Suresh ; Alagumanikumaran, N. ; Kaviyarasu, K. ; Muthukumaran, Azhaguchamy |
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Zeitschrift: | Journal of Nanoparticle Research, Jg. 26 (2024-03-01), Heft 3, S. 1-16 |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 1388-0764 (print) |
DOI: | 10.1007/s11051-024-05956-x |
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