Targeted DNA N<superscript>6</superscript>‐methyladenine editing by dCas9 fused to METTL4 in the lepidopteran model insect Bombyx mori.

A recent article in the journal Insect Science discusses the development of a new CRISPR-dCas9-METTL4 epigenome editing tool that can methylate target regions to achieve site-specific DNA 6mA methylation in the silkworm Bombyx mori. The study investigates the role of 6mA DNA modification in regulating gene expression and explores the molecular mechanism of 6mA in target gene expression. The researchers successfully constructed a dCas9-METTL4 fusion protein and demonstrated its ability to induce site-specific methylation on DNA adenines. The study provides valuable insights into the epigenetic regulation of gene expression through site-specific 6mA modification in cultured silkworm cells. [Extracted from the article]