Activating the healing process: three-dimensional culture of stem cells in Matrigel for tissue repair.
In: BMC Biotechnology, Jg. 24 (2024-05-25), Heft 1, S. 1-13
Online
academicJournal
Zugriff:
Background: To establish a strategy for stem cell-related tissue regeneration therapy, human gingival mesenchymal stem cells (hGMSCs) were loaded with three-dimensional (3D) bioengineered Matrigel matrix scaffolds in high-cell density microtissues to promote local tissue restoration. Methods: The biological performance and stemness of hGMSCs under 3D culture conditions were investigated by viability and multidirectional differentiation analyses. A Sprague‒Dawley (SD) rat full-thickness buccal mucosa wound model was established, and hGMSCs/Matrigel were injected into the submucosa of the wound. Autologous stem cell proliferation and wound repair in local tissue were assessed by histomorphometry and immunohistochemical staining. Results: Three-dimensional suspension culture can provide a more natural environment for extensions and contacts between hGMSCs, and the viability and adipogenic differentiation capacity of hGMSCs were significantly enhanced. An animal study showed that hGMSCs/Matrigel significantly accelerated soft tissue repair by promoting autologous stem cell proliferation and enhancing the generation of collagen fibers in local tissue. Conclusion: Three-dimensional cell culture with hydrogel scaffolds, such as Matrigel, can effectively improve the biological function and maintain the stemness of stem cells. The therapeutic efficacy of hGMSCs/Matrigel was confirmed, as these cells could effectively stimulate soft tissue repair to promote the healing process by activating the host microenvironment and autologous stem cells. [ABSTRACT FROM AUTHOR]
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Activating the healing process: three-dimensional culture of stem cells in Matrigel for tissue repair.
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Autor/in / Beteiligte Person: | Xu, Shukui ; Zhao, Liru ; Li, Yinghui ; Gu, Xiuge ; Liu, Ziyang ; Han, Xing ; Li, Wenwen ; Ma, Wensheng |
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Zeitschrift: | BMC Biotechnology, Jg. 24 (2024-05-25), Heft 1, S. 1-13 |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 1472-6750 (print) |
DOI: | 10.1186/s12896-024-00862-5 |
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