Genetic diversity of the gene pool of bread winter wheat alleles of microsatellite locus Xgwm182-5D associated with frost resistance.

Aim. Identification of winter wheat varieties from different regions at Xgwm182-5D locus. Methods. Polymerase chain reaction (PCR), gelelectrophoresis. Results. 180 genotypes of winter wheat varieties from Ukraine and Russia selection centers are identified by the locus Xgwm182-5D. There were detected five alleles of this locus, namely 162, 165, 167, 169 and 174 bp. Allele 165 bp was noted to be more common in the sets of investigated varieties in all regions. Allele 162 bp was identified only in the sets of varieties of Southern Ukraine and North Caucasus (20%and 4%, respectively). Allele 169 bp was detected only in varieties of Western Siberia and Volga region. Conclusions. The differences in frequencies of locus Xgwm182-5D alleles may be due to adaptive and breeding value of these alleles for the conditions of certain regions. [ABSTRACT FROM AUTHOR]