Isolation and complete genomic characterization of a Movar 33/63-like Japanese bovine herpesvirus 4 from a calf with respiratory disease.
In: Journal of Veterinary Medical Science, Jg. 86 (2024-05-01), Heft 5, S. 645-652
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Zugriff:
Bovine herpesvirus 4 (BoHV-4) is an indigenous virus in cattle prevalent mainly in North and South American countries and European countries, but the genomic sequences and genetic characteristics of Japanese strains have not been reported. BoHV-4 is suspected, but not proven, to be associated with various diseases. In the present study, we isolated BoHV-4 from a 10-month-old Japanese Black calf with respiratory symptoms in Japan. To identify the genetic characteristics of the isolate named strain SG20, complete genome sequencing was performed using a combination of next-generation and Sanger sequencing technologies. The complete long unique coding region (LUR) of SG20 was found to comprise 108,819 nucleotides with 41.4% GC content and contain at least 78 open reading frames. It shares 83.4 to 99.3% overall nucleotide identity with six BoHV-4 strains available in the database. The deduced amino acid sequence alignment revealed that SG20 contains genotype 1-specific features of BoHV-4, such as amino acid substitutions and insertions within the glycoprotein B region. Phylogenetic analyzes based on the nucleotide sequences of ORF20 indicated that the virus belonged to genotype 1 (Movar 33/63-like group). The strain was also analyzed using the complete LUR and placed in the same clade as a strain recently isolated from China, but it was distinct from American and European BoHV-4 strains of genotype 1. Although further genomic and epidemiologic information is needed, our results help elucidate the molecular epidemiology of BoHV-4 and provide a foundation for future studies. [ABSTRACT FROM AUTHOR]
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Titel: |
Isolation and complete genomic characterization of a Movar 33/63-like Japanese bovine herpesvirus 4 from a calf with respiratory disease.
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Autor/in / Beteiligte Person: | Asuka, KUMAGAI ; Yasufumi, SOGA ; Kumiko, KIMURA ; Shinichi, HATAMA |
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Zeitschrift: | Journal of Veterinary Medical Science, Jg. 86 (2024-05-01), Heft 5, S. 645-652 |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 0916-7250 (print) |
DOI: | 10.1292/jvms.24-0028 |
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