Porcine deltacoronavirus nonstructural protein 2 inhibits type I and III IFN production by targeting STING for degradation.
In: Veterinary Research, Jg. 55 (2024-06-17), Heft 1, S. 1-14
Online
academicJournal
Zugriff:
Porcine deltacoronavirus (PDCoV) is an enteropathogenic coronavirus that has been reported to use various strategies to counter the host antiviral innate immune response. The cGAS-STING signalling pathway plays an important role in antiviral innate immunity. However, it remains unclear whether PDCoV achieves immune evasion by regulating the cGAS-STING pathway. Here, we demonstrated that the nonstructural protein 2 (nsp2) encoded by PDCoV inhibits cGAS-STING-mediated type I and III interferon (IFN) responses via the regulation of porcine STING (pSTING) stability. Mechanistically, ectopically expressed PDCoV nsp2 was found to interact with the N-terminal region of pSTING. Consequently, pSTING was degraded through K48-linked ubiquitination and the proteasomal pathway, leading to the disruption of cGAS-STING signalling. Furthermore, K150 and K236 of pSTING were identified as crucial residues for nsp2-mediated ubiquitination and degradation. In summary, our findings provide a basis for elucidating the immune evasion mechanism of PDCoV and will contribute to the development of targets for anti-coronavirus drugs. [ABSTRACT FROM AUTHOR]
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Titel: |
Porcine deltacoronavirus nonstructural protein 2 inhibits type I and III IFN production by targeting STING for degradation.
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Autor/in / Beteiligte Person: | Liu, Xiqian ; Ji, Likai ; Cheng, Yuqiang ; Kong, Linghe ; Xie, Songhua ; Yang, Juan ; Chen, Jiaqi ; Wang, Zhaofei ; Ma, Jingjiao ; Wang, Hengan ; Yan, Yaxian ; Sun, Jianhe |
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Zeitschrift: | Veterinary Research, Jg. 55 (2024-06-17), Heft 1, S. 1-14 |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 0928-4249 (print) |
DOI: | 10.1186/s13567-024-01330-w |
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