Generation of human iPSC-derived neurofibromaspheres for in vitro and in vivo uses
In: STAR Protocols, Jg. 4 (2023), Heft 2, S. 102198-
Online
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Summary: Neurofibromas are benign peripheral nervous system tumors associated with neurofibromatosis type 1, which originate from NF1(−/−) Schwann cell precursors. We describe a protocol to generate neurofibromaspheres by differentiating NF1(−/−) Schwann cells from induced pluripotent stem cells and combining them with neurofibroma primary fibroblasts. We also describe the development of neurofibroma-like tumors when neurofibromaspheres are engrafted in the sciatic nerve of nude mice. This model constitutes a versatile platform for drug screening and the study of neurofibroma biology.For complete details on the use and execution of this protocol, please refer to Mazuelas et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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Generation of human iPSC-derived neurofibromaspheres for in vitro and in vivo uses
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Autor/in / Beteiligte Person: | Mazuelas, Helena ; Uriarte-Arrazola, Itziar ; Fernández-Rodríguez, Juana ; Magallón-Lorenz, Miriam ; Villanueva, Alberto ; Lázaro, Conxi ; Gel, Bernat ; Serra, Eduard ; Carrió, Meritxell |
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Zeitschrift: | STAR Protocols, Jg. 4 (2023), Heft 2, S. 102198- |
Veröffentlichung: | Elsevier, 2023 |
Medientyp: | academicJournal |
ISSN: | 2666-1667 (print) |
DOI: | 10.1016/j.xpro.2023.102198 |
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