Cannabis sativa L. essential oil: Chemical composition, anti-oxidant, anti-microbial properties, and acute toxicity: In vitro, in vivo, and in silico study
In: Open Chemistry, Jg. 22 (2024), Heft 1, S. 96-111
Online
academicJournal
Zugriff:
This study evaluated the volatile components of Cannabis sativa L. essential oils (CSEOs) and their pharmacological potential in vitro, in animal, and in silico. The anti-oxidant capacities of volatile compounds were tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), total anti-oxidant capacity (TAC), and gas chromatography-mass spectrometry (GC-MS). Anti-microbial activity against bacterial and fungal strains was assessed using disk diffusion and micro-dilution, and acute toxicity was examined on mice using OECD 423 criteria. The results indicate that the main components were β-caryophyllene (31.54%), α-humulene (12.62%), β-myrcene (4.83%), and α-pinene (4.69%). The essential oil showed high anti-oxidant ability (IC50 = 0.981 ± 0.059 mg/ml for DPPH, EC50 = 1.74 ± 0.05 for FRAP), and TAC of 0.101 ± 0.001 mg AAE/g. Additionally, it showed significant antibacterial action against Gram-negative organisms, such as Escherichia coli (11.33 ± 0.00 mm), Klebsiella pneumonia (9 ± 0.00 mm), and Pseudomonas aeruginosa (9.34 ± 0.00), with MICs ranging from 0.0052 to 0.0208 mg/CSEO demonstrated antifungal activity against Candida albicans and Fusarium proliferatum, with activity levels of 18.66 ± 0.88 mm, 41.89 ± 3.60%, and MICs of 0.39 and 0.013 mg/ml, respectively. In toxicological studies, CSEO proved to be safe for animals. Docking identified bioactive components and explored anti-oxidant and antibacterial properties. Docking proved that bulnesol and champacol caused indicated actions.
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Cannabis sativa L. essential oil: Chemical composition, anti-oxidant, anti-microbial properties, and acute toxicity: In vitro, in vivo, and in silico study
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Autor/in / Beteiligte Person: | Rafik, El-Mernissi ; El Menyiy Naoual ; Rania, Moubachir ; Aziz, Zouhri ; Yahya, El-Mernissi ; Farhan, Siddique ; Sumaira, Nadeem ; Hind, Ibork ; El Barnossi Azeddin ; Wondmie Gezahign Fentahun ; Mohammed, Bourhia ; Bin Jardan Yousef A. ; Oualid, Abboussi ; Lhoussain, Hajji |
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Zeitschrift: | Open Chemistry, Jg. 22 (2024), Heft 1, S. 96-111 |
Veröffentlichung: | De Gruyter, 2024 |
Medientyp: | academicJournal |
ISSN: | 2391-5420 (print) ; 2023-0214 (print) |
DOI: | 10.1515/chem-2023-0214 |
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