C37H53FO3, orthorhombic, P212121 (no. 19), a = 7.4716(
Keywords: 2283345
The molecular structure is shown in the figure. Table 1 contains crystallographic data and Table 2 contains the list of the atoms including atomic coordinates and displacement parameters.
Graph
Table 1: Data collection and handling.
Crystal: Colourless block Size: 0.15 × 0.13 × 0.12 mm Wavelength: Mo Diffractometer, scan mode: SuperNova, 10139, 5655, 0.057 Criterion for 379 Programs: CrysAlisPRO [
Table 2: Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å
Atom C1 0.6411 (6) 0.0540 (3) 0.25259 (16) 0.0250 (10) C2 0.5605 (6) −0.0356 (3) 0.24405 (16) 0.0246 (10) C3 0.3790 (6) −0.0434 (3) 0.21838 (17) 0.0274 (11) C4 0.3220 (5) 0.0443 (3) 0.19277 (16) 0.0222 (10) H4 0.256298 0.078136 0.216962 0.027* C5 0.4772 (5) 0.1065 (3) 0.17742 (16) 0.0197 (10) C6 0.5760 (6) 0.1325 (3) 0.22391 (17) 0.0283 (11) H6A 0.677847 0.169683 0.215817 0.034* H6B 0.495993 0.167770 0.243537 0.034* C7 0.1863 (6) 0.0282 (3) 0.15306 (17) 0.0267 (11) H7A 0.244156 −0.003253 0.127091 0.032* H7B 0.090440 −0.009221 0.165024 0.032* C8 0.1078 (5) 0.1146 (2) 0.13440 (16) 0.0207 (10) H8A 0.038396 0.142394 0.159554 0.025* H8B 0.026801 0.101110 0.108379 0.025* C9 0.2489 (5) 0.1810 (2) 0.11684 (15) 0.0166 (9) C10 0.3940 (5) 0.1937 (3) 0.15613 (16) 0.0186 (9) H10 0.330666 0.221638 0.182805 0.022* C11 0.1636 (5) 0.2755 (3) 0.10745 (15) 0.0181 (9) C12 0.3077 (5) 0.3404 (2) 0.08845 (15) 0.0166 (9) H12 0.361478 0.312149 0.060422 0.020* C13 0.4573 (5) 0.3530 (3) 0.12480 (16) 0.0221 (10) H13 0.409607 0.384209 0.152726 0.027* C14 0.5332 (6) 0.2627 (3) 0.14047 (16) 0.0222 (10) H14A 0.601597 0.237894 0.114257 0.027* H14B 0.615396 0.272611 0.166686 0.027* C15 0.0239 (5) 0.2811 (3) 0.06694 (16) 0.0226 (10) H15A 0.059275 0.244497 0.040070 0.027* H15B −0.092950 0.261901 0.077960 0.027* C16 0.0214 (6) 0.3796 (3) 0.05330 (17) 0.0240 (10) H16A −0.078830 0.409407 0.068463 0.029* H16B 0.009225 0.386020 0.019062 0.029* C17 0.2014 (5) 0.4217 (2) 0.07037 (16) 0.0186 (9) H17 0.174667 0.459274 0.098014 0.022* C18 0.2874 (5) 0.4814 (3) 0.03234 (16) 0.0206 (10) C19 0.1547 (6) 0.5498 (3) 0.01323 (17) 0.0231 (10) H19A 0.071774 0.520280 −0.008175 0.028* H19B 0.086120 0.573913 0.039603 0.028* C20 0.2452 (6) 0.6262 (3) −0.01321 (18) 0.0298 (11) H20A 0.304118 0.603528 −0.041517 0.036* H20B 0.155940 0.669366 −0.023118 0.036* C21 0.3815 (6) 0.6711 (3) 0.01873 (18) 0.0309 (11) H21A 0.319665 0.700109 0.044862 0.037* H21B 0.443573 0.716795 0.000679 0.037* C22 0.5180 (6) 0.6062 (3) 0.03884 (19) 0.0323 (12) C23 0.6146 (8) 0.6442 (3) 0.0817 (2) 0.0564 (17) H23A 0.529313 0.657967 0.106225 0.085* H23B 0.676983 0.697505 0.072639 0.085* H23C 0.698840 0.601177 0.093503 0.085* C24 0.3296 (6) 0.1431 (3) 0.07029 (16) 0.0243 (10) H24A 0.343066 0.079721 0.073180 0.036* H24B 0.444465 0.169828 0.064707 0.036* H24C 0.251319 0.156366 0.044107 0.036* C25 0.6128 (6) 0.0601 (3) 0.14458 (17) 0.0281 (11) H25A 0.693058 0.103594 0.131454 0.042* H25B 0.550274 0.030527 0.119208 0.042* H25C 0.679782 0.017046 0.162561 0.042* C26 0.3873 (7) −0.1254 (3) 0.18501 (19) 0.0366 (13) H26A 0.465577 −0.112776 0.158722 0.055* H26B 0.269586 −0.138391 0.173220 0.055* H26C 0.432035 −0.175815 0.202369 0.055* C27 0.6551 (6) 0.5790 (3) 0.0012 (2) 0.0503 (16) H27A 0.720333 0.527817 0.012198 0.075* H27B 0.736597 0.627377 −0.004220 0.075* H27C 0.594464 0.564600 −0.027936 0.075* C28 0.0703 (5) 0.3142 (3) 0.15232 (15) 0.0197 (10) H28A 0.156058 0.319918 0.177599 0.030* H28B −0.024115 0.274842 0.162142 0.030* H28C 0.021163 0.371697 0.144972 0.030* C29 0.2456 (7) −0.0632 (3) 0.2587 (2) 0.0415 (14) H29A 0.271794 −0.120487 0.272305 0.062* H29B 0.126071 −0.063334 0.246192 0.062* H29C 0.255695 −0.018190 0.282894 0.062* C30 0.7624 (6) 0.0587 (3) 0.28762 (16) 0.0243 (10) H30 0.782540 0.006029 0.304246 0.029* C31 0.8686 (6) 0.1364 (3) 0.30341 (16) 0.0223 (10) C32 0.8743 (6) 0.1573 (3) 0.35243 (16) 0.0271 (10) H32 0.809143 0.124435 0.374530 0.033* C33 0.9780 (6) 0.2271 (3) 0.36631 (17) 0.0310 (11) C34 1.0831 (6) 0.2757 (3) 0.33611 (18) 0.0309 (11) H34 1.154299 0.321914 0.347458 0.037* C35 1.0807 (6) 0.2543 (3) 0.28823 (18) 0.0345 (12) H35 1.151275 0.285739 0.266717 0.041* C36 0.9725 (6) 0.1858 (3) 0.27274 (17) 0.0277 (11) H36 0.969566 0.172529 0.240384 0.033* C37 0.3677 (6) 0.4253 (3) −0.00826 (16) 0.0315 (11) H37A 0.389003 0.462763 −0.035422 0.047* H37B 0.285598 0.378782 −0.016783 0.047* H37C 0.478640 0.399502 0.002094 0.047* F1 0.9792 (4) 0.24862 (19) 0.41389 (10) 0.0484 (8) O1 0.4259 (4) 0.52890 (18) 0.05861 (11) 0.0257 (7) O2 0.6064 (4) 0.40139 (19) 0.10659 (12) 0.0311 (8) H2 0.573599 0.451070 0.098096 0.047* O3 0.6373 (4) −0.10299 (18) 0.25643 (12) 0.0340 (8)
Total saponins from ginseng stems and leaves were added to an ethanol solution of 18 % sulfuric acid and heated to reflux for 4 h. The reaction solution was cooled to room temperature, appropriate amount of sodium carbonate was added to neutralize sulfuric acid, the reaction solution was adjusted to pH 7. The residue was extracted with ethyl acetate, washed with saturated brine, dried with anhydrous sodium sulfate, filtered, concentrated under reduced pressure to obtain the crude product, and the crude product was recrystallized with ethyl acetate to obtain the pure product of 20(R)-panaxadiol. 20(R)-panaxadiol and PCC (pyridinium chlorochromate) were dissolved in 25 mL of dichloromethane, and the reaction mixture was stirred at room temperature for 4 h. The intermediate 20(R)-3-oxopanaxadiol was purified by silica gel column chromatography. Methanol (1.4 mL), 20(R)-3-oxopanaxadiol (100 mg, 0.22 mmol), and 2-fluorobenzaldehyde (0.023 mL, 0.22 mmol) were added to a round-bottomed flask, to which 0.72 mL (25 %) aqueous sodium hydroxide was added dropwise, and the flask was stirred for 4 h at room temperature. The reaction was monitored by thin layer chromatography (TLC, 254 nm). At the end of the reaction, appropriate amount of water was added, the reaction mixture was transferred to a partition funnel and extracted with ethyl acetate (twice), the organic phases were combined and washed with saturated sodium chloride solution, dried with anhydrous sodium sulfate, filtered, concentrated under reduced pressure and the crude product was purified by silica gel column chromatography. Recrystallization with ethyl acetate solution gave crystals of the target compound.
The H atoms were placed in idealized positions and treated as riding on their parent atoms [[
Ginseng saponins include protopanoxadiol-type saponin, panaxatriol-type saponin and oleanane-type saponin, sharing a tetrahydrofuran ring and a dammarane skeleton [[
The ORTEP diagram is presented in the Figure. The title compound contains one drug molecule in the asymmetric unit (cf. the figure). Bond lengths and angles are all in the expected ranges. The title compound has a dammarane type as the parent structure [[
This work was supported by Science and Technology Innovation Development Plan of Yantai (No. 2020XDRH105) and the National Natural Science Foundation of China (No. 81473104).
By Kuo Cheng; Ya-Bin Song; Qing-Guo Meng; Bao-Gang Wang; Yun Hou; Wen-Shui Wang and Sheng Jiang
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