Limbal stem cells carried by a four-dimensional -printed chitosan-based scaffold for corneal epithelium injury in diabetic rabbits
In: Frontiers in Physiology, Jg. 15 (2024-06-01)
Online
academicJournal
Zugriff:
Methods: Herein, we obtained and characterized deltaN p63- and adenosine triphosphate-binding cassette subfamily G member 2-expressing limbal stem cells (LSCs). Chitosan and carboxymethyl chitosan (CTH) were cross-linked to be an in situ thermosensitive hydrogel (ACH), which was printed through four-dimensional (4D) printing to obtain a porous carrier with uniform pore diameter (4D-CTH). Rabbits were injected with alloxan to induce diabetes mellitus (DM). Following this, the LSC-carrying hydrogel was spread on the surface of the cornea of the diabetic rabbits to cure corneal epithelium injury.Results: Compared with the control group (LSCs only), rapid wound healing was observed in rabbits treated with LSC-carrying 4D-CTH. Furthermore, the test group also showed better corneal nerve repair ability. The results indicated the potential of LSC-carrying 4D-CTH in curing corneal epithelium injury.Conclusion: 4D-CTH holds potential as a useful tool for studying regenerative processes occurring during the treatment of various diabetic corneal epithelium pathologies with the use of stem cell-based technologies.
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Limbal stem cells carried by a four-dimensional -printed chitosan-based scaffold for corneal epithelium injury in diabetic rabbits
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Autor/in / Beteiligte Person: | Wang, Mengyuan ; Liu, Kaibin ; Wang, Xiaomin ; Shang, Zhen ; Liu, Yiming ; Pan, Nailong ; Sun, Xueqing ; Xu, Wenhua |
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Zeitschrift: | Frontiers in Physiology, Jg. 15 (2024-06-01) |
Veröffentlichung: | Frontiers Media S.A., 2024 |
Medientyp: | academicJournal |
ISSN: | 1664-042X (print) |
DOI: | 10.3389/fphys.2024.1285850 |
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