Live imaging and quantitation of insect feeding-induced Ca2+ signal using GCaMP3-based system in Nicotiana benthamiana
In: STAR Protocols, Jg. 3 (2022), Heft 1, S. 101040-
Online
academicJournal
Zugriff:
Summary: Wounding evokes transient increases in cytosolic calcium (Ca2+) concentration. Visualizing real-time Ca2+ flux provides new insights into Ca2+-signaling pathways. Here, we outline a protocol to detect insect feeding-induced Ca2+ flux elevation in Nicotiana benthamiana leaves based on the GCaMP3 reporter system by Leica fluorescence stereo microscopes (LFSM). LFSM combines super-fast manual screening with high-end imaging capabilities. Through this protocol, we can clearly observe the calcium flow after aphid’s piercing-sucking. Additionally, we describe a protocol to quantify Ca2+ level using LFSM.For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).
Titel: |
Live imaging and quantitation of insect feeding-induced Ca2+ signal using GCaMP3-based system in Nicotiana benthamiana
|
---|---|
Autor/in / Beteiligte Person: | Wang, Yunjing ; Gong, Qian ; Huang, Fan ; He, Linfang ; Liu, Yule |
Link: | |
Zeitschrift: | STAR Protocols, Jg. 3 (2022), Heft 1, S. 101040- |
Veröffentlichung: | Elsevier, 2022 |
Medientyp: | academicJournal |
ISSN: | 2666-1667 (print) |
DOI: | 10.1016/j.xpro.2021.101040 |
Schlagwort: |
|
Sonstiges: |
|