Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice
In: eLife, Jg. 13 (2024-05-01)
Online
academicJournal
Zugriff:
The flow of neural activity across the neocortex during active sensory discrimination is constrained by task-specific cognitive demands, movements, and internal states. During behavior, the brain appears to sample from a broad repertoire of activation motifs. Understanding how these patterns of local and global activity are selected in relation to both spontaneous and task-dependent behavior requires in-depth study of densely sampled activity at single neuron resolution across large regions of cortex. In a significant advance toward this goal, we developed procedures to record mesoscale 2-photon Ca2+ imaging data from two novel in vivo preparations that, between them, allow for simultaneous access to nearly all 0f the mouse dorsal and lateral neocortex. As a proof of principle, we aligned neural activity with both behavioral primitives and high-level motifs to reveal the existence of large populations of neurons that coordinated their activity across cortical areas with spontaneous changes in movement and/or arousal. The methods we detail here facilitate the identification and exploration of widespread, spatially heterogeneous neural ensembles whose activity is related to diverse aspects of behavior.
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Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice
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Autor/in / Beteiligte Person: | Evan D Vickers ; David A McCormick |
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Zeitschrift: | eLife, Jg. 13 (2024-05-01) |
Veröffentlichung: | eLife Sciences Publications Ltd, 2024 |
Medientyp: | academicJournal |
ISSN: | 2050-084X (print) |
DOI: | 10.7554/eLife.94167 |
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